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Jehovah’s Witnesses have been especially harshly treated since independence. There is no truth to the statement in the Eritrean report to the Commission (on p19) that Eritrean Jehovah’s Witnesses refused to recognize the government and “opposed the referendum process” – rather they declined to participate in the 1993 referendum on independence solely because of their beliefs. Members of the faith also refused to serve as soldiers in national service because of conscientious objections. Eritrea provides no substitute service for conscientious objectors. Instead, the government has imprisoned Jehovah’s Witnesses, young and old, and denied them ration cards and work permits. Fifty-four are currently in detention, including three arrested and sent to the Sawa military training camp 24 years ago. Prison conditions for Jehovah’s Witnesses improved somewhat in 2017. All Witness prisoners, including the Sawa-three, were transferred to the Mai Serwa prison last year. There, they have been allowed visitors for the first time during incarceration and conditions are said to be less oppressive.

“Recognized” religions are hardly immune from government repression. The government deposed Eritrean Orthodox Patriarch Antonios in 2007, placed him under house arrest, and imposed a successor on the church. In July 2017, the octogenarian former patriarch was brought to a church service for the first time in 11 years but not allowed to speak. He has not been seen since. The government also appointed the Mufti of the Muslim community. Religious leaders and laymen who protested the patriarchal and mufti appointments remain imprisoned.

[1] Zegveld v. Eritrea, African Commission on Human and People’s Rights, 35th Ordinary Session, “Seventeenth Annual Activity, Report of the African Commission on Human and Peoples’ Rights 2003–2004,” June 2004, Banjul, Gambia, Jack Rogers Sparkle Georgica Jelly Sandal k5CS51q
. Zegveld v. Eritrea,” communication 250/2002, was adopted at the 34th Ordinary Session and issued in Annex VI.

Article 19 v. State of Eritrea, African Commission on Human and People’s Rights,11th Ordinary Session, “Twenty-Second Activity Report of the African Commission on Human and Peoples’ Rights,” Accra, Ghana, June 2007, http://www.achpr.org/english/activity_reports/activty22_eng.pdf . Article 19 v. State of Eritrea,” communication 275/2003, was adopted at the 41st Ordinary Session in May 2007 and issued as Annex II.

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FIGURE 6.

Stable knockdown of SLFN2 enhances cell proliferation and impairs IFNα-dependent growth inhibitory responses but has no effects on the generation of antiviral responses. , expression of or mRNAs in pSIREN Zsgreen-SLFN2 siRNA and pSIREN Zsgreen control-siRNA NIH3T3 cells was determined by real time RT-PCR using specific primers and as an internal control. The data are presented as percentages of expression in pSIREN Zsgreen control-siRNA cells and represent the means ± S.E. of three experiments. , total cell lysates from pSIREN Zsgreen SLFN2-siRNA or pSIREN Zsgreen control-siRNA NIH3T3 cells were resolved by SDS-PAGE and immunoblotted sequentially with anti-SLFN2 or anti-GAPDH antibodies. , equal numbers of pSIREN Zsgreen SLFN2-siRNA or pSIREN Zsgreen control-siRNA NIH3T3 cells were plated and were left untreated or were treated with the indicated doses of mouse IFNα. After 5–7 days cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assays. A representative experiment is shown in the The means ± S.E. of three experiments, including the one shown in the picture on the , are shown on the and , pSIREN Zsgreen SLFN2 siRNA or pSIREN Zsgreen control-siRNA NIH3T3 cells were treated with IFNα for 15 min, as indicated. Nuclear extracts were reacted with 40,000 cpm of P-labeled ISRE () or SIE () oligonucleotides, and complexes were resolved by native gel electrophoresis and visualized by autoradiography. The migration of the different STAT complexes is indicated by , wild-type NIH3T3 cells, pSIREN Zsgreen SLFN2 siRNA NIH3T3 cells, or pSIREN Zsgreen control-siRNA NIH3T3 cells were treated with IFNα for the indicated times. Expression of mRNA was determined by real time RT-PCR using as an internal control. The data are expressed as the means ± S.E. of three experiments. , wild-type NIH3T3 cells, pSIREN Zsgreen SLFN2-siRNA NIH3T3 cells, or pSIREN Zsgreen control-siRNA NIH3T3 cells were incubated in triplicate, in the presence or absence of the indicated concentrations of IFNα. The cells were subsequently challenged with encephalomyocarditis virus (), and cytopathic effects were quantified 24 h later. The data are expressed as percentages of protection from the cytopathic effects of encephalomyocarditis virus. A representative of three independent experiments is shown.

To examine whether SLFN2 plays a role in the control of anchorage-independent growth, we assayed transduced NIH3T3 cells for colony formation in soft agar ( 41 ). Colony formation was clearly increased in NIH3T3 pSIREN Zsgreen SLFN2-siRNA cells as compared with NIH3T3 pSIREN Zsgreen Ctrl siRNA cells ( Gabor Gabor 64340 SWAa2ROSF
, A and B ). Notably, the colonies from NIH3T3 pSIREN Zsgreen SLFN2-siRNA cells were consistently larger ( Fig. 7 A ), and the numbers of colonies were increased ( Fig. 7 B ) as compared with NIH3T3 pSIREN Zsgreen Ctrl siRNA cells. Taken altogether, these data for the first time implicate SLFN2 in the regulation of anchorage-independent growth.

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